Journal: eBioMedicine
Article Title: Renal podocyte senescence in human obesity detected by urinary extracellular vesicles
doi: 10.1016/j.ebiom.2025.106116
Figure Lengend Snippet: Correlation between P16 + MCP-1 + PODXL + podocyte-derived uEVs and clinical parameters . The proportion of P16 + MCP-1 + PODXL + uEVs in HV (blue, n = 10) and OB (red, n = 21) was significantly positively correlated with (A) BMI, (B) HOMA-IR, (C) insulin, (D) and urinary (U) levels of KIM-1, (E) NGAL, (F) protein, and (G) TNF-α; BMI, body mass index; HOMA-IR, homoeostatic model assessment for insulin resistance; A trend line is shown to illustrate the overall directionality of the association. KIM-1, kidney injury molecule-1; NGAL, neutrophil gelatinase-associated lipocalin; TNF-α, tumour necrosis factor-alpha.
Article Snippet: Additional biomarkers were quantified using commercially available enzyme-linked immunosorbent assays, including urinary neutrophil gelatinase-associated lipocalin (NGAL; ThermoFisher, Cat#KIT036), kidney injury molecule-1 (KIM-1; R&D Systems, Minneapolis, MN, Cat#DKM100), insulin (Sigma–Aldrich, Saint Louis, MO, Cat#RAB0327), tumour necrosis factor-alpha (TNF-α; Sigma–Aldrich, Cat#RAB0476), and monocyte chemoattractant protein-1 (MCP-1; R&D, Cat#DCP00).
Techniques: Derivative Assay